Site Directed Mutagenesis (SDM) Human Insertion IMR-32

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Rat primary vascular smooth muscle cells Biotin

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat aortic smooth muscle cells

Get tips on using IRIS11 Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using IRIS9 Plus Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using pLV-EF1a-IRES-Puro to perform CRISPR Mouse - Deletion L929 Ppm1b

Get tips on using IRDye® 800CW Streptavidin to perform Western blot Biotin - -NA- -NA- Infrared dye 800CW

Get tips on using IRIS9 Prestained Protein Ladder（15 to 180 kDa） to perform Protein Ladder Prestained

Get tips on using pFastBac-GP67-H6HA1-His-RhPV-IRES-EGFP to perform Protein Expression Eukaryotic cells - S. frugiperda HA1 of H6N1 AIV

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven