rna-isolation-purification-cells-immortalized-cal-27

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - MCF7

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - A172

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - A375

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - CAMs

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - CAMs

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - CLL

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - HEK 293