Immunohistochemistry Collagen Type III Goat

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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type U2OS (human bone osteosarcoma epithelial cells)

Get tips on using Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Products Cell Signaling Technology Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660

Get tips on using NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB to perform Immunohistochemistry Human - Naspsin A

Products IBL, Immuno-Biological Laboratories co,Ltd NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB

Get tips on using Anti-Beta Catenin CTNNB1 Antibody Picoband™ (Monoclonal, 1F6) to perform Immunohistochemistry Human - β-catenin

Products BosterBio Anti-Beta Catenin CTNNB1 Antibody Picoband™ (Monoclonal, 1F6)

Get tips on using NF-κB p65 (D14E12) XP® Rabbit mAb #8242 to perform Immunohistochemistry Mouse - NFκB / p65

Products Cell Signaling Technology NF-κB p65 (D14E12) XP® Rabbit mAb #8242

Get tips on using OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY to perform Immunohistochemistry Mouse - Spp1/OPN

Products IBL, Immuno-Biological Laboratories co,Ltd OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Products Abcam Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572)

Get tips on using Purified Mouse Anti-Human MSH-2 Clone G219-1129 (RUO) to perform Immunohistochemistry Human - MSH2

Products BD Biosciences Purified Mouse Anti-Human MSH-2 Clone G219-1129 (RUO)

Get tips on using Monoclonal Mouse Anti-Villin (Autostainer Link 48) Clone 1D2 C3 to perform Immunohistochemistry Human - Villin

Products Agilent Technologies Monoclonal Mouse Anti-Villin (Autostainer Link 48) Clone 1D2 C3

Get tips on using Monoclonal Mouse Anti-Human Progesterone Receptor (Concentrate) Clone PgR 1294 to perform Immunohistochemistry Human - PR

Products Agilent Technologies Monoclonal Mouse Anti-Human Progesterone Receptor (Concentrate) Clone PgR 1294

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