Immunohistochemistry chk2 phospho (Thr 68) Rabbit IgG Human

Get tips on using Anti-Collagen Type II Antibody, clone 6B3 to perform Immunohistochemistry Mouse - Col II

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 to perform Immunohistochemistry Rat - GFAP

Get tips on using Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) to perform Immunohistochemistry Mouse - TUBB3

Get tips on using Estrogen Receptor alpha Antibody (F-10): sc-8002 to perform Immunohistochemistry Mouse - ERα

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Rat GFR alpha-1/GDNF R alpha-1 Antibody to perform Immunohistochemistry Mouse - GFRA1

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24