Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hPSC or hiPSCS differentiation into myogenic cells
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Fenozol to perform Stem cell Differentiation media Differentiation of Human iPSCs into Basal Forebrain cholinergic neurons (BFCN)
Get tips on using Gibco™IMDM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)
Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hiPSCs or hPSCs differentiation into trophoblasts
Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)
Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)
Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage
Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage
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