Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Get tips on using pET21b-TH8G to perform Protein Expression Prokaryotic cells - E. coli TH8G

Get tips on using pET21b-TH6G to perform Protein Expression Prokaryotic cells - E. coli TH6G

Get tips on using pET21b-TH4G to perform Protein Expression Prokaryotic cells - E. coli TH4G

Get tips on using pET21b-TH2G to perform Protein Expression Prokaryotic cells - E. coli TH2G

Get tips on using Mouse MPO/Myeloperoxidase PicoKine™ ELISA Kit Skip to the end of the images gallery to perform ELISA Mouse - MPO

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using THIOGLYCOLLATE BROTH USP to perform Bacterial cell culture media Clostridium tetani