Protein Expression Eukaryotic cells S. frugiperda

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Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation HEK293 MDR3

Products Thermo Fisher Scientific Phusion Site-Directed Mutagenesis Kit

Get tips on using 1 kb Plus DNA Ladder for Safe Stains to perform DNA Ladder 1 kb

Products New England BioLabs 1 kb Plus DNA Ladder for Safe Stains

Get tips on using ON-TARGETplus Human PPARGC1B siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PGC-1β/PPARGC1B

Products Horizon Discovery Ltd. ON-TARGETplus Human PPARGC1B siRNA

Get tips on using PI 3-kinase p100 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B PIK3C3

Products Santa Cruz Biotechnology PI 3-kinase p100 siRNA (h)

Get tips on using 14-3-3ζ siRNA(h) to perform siRNA / miRNA gene silencing Human - Caco-2 14‐3‐3ζ

Products Santa Cruz Biotechnology 14-3-3ζ siRNA(h)

Get tips on using QuikChange Lightning Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion L929 ICP6

Products Agilent Technologies QuikChange Lightning Site-Directed Mutagenesis Kit

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR - fibroblast

Products Bioline SensiFAST™ Probe No-ROX One-Step Kit

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Monkey - Point mutation Cos-7 GARS

Products Thermo Fisher Scientific Phusion Site-Directed Mutagenesis Kit

Get tips on using HiPurA™ Streptomyces DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Products HiMEDIA HiPurA™ Streptomyces DNA Purification Kit

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

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