RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using Targefect-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using pCW-LIC to perform Protein expression and purification Bacteria - Escherichia coli Fbxo7

Get tips on using exoEasy Maxi Kit (20) to perform Purification of extracellular vesicles Exosomes - Plasma

Get tips on using exoRNeasy Midi Kit (50) to perform Purification of extracellular vesicles Exosomes - Plasma

Get tips on using jetPEI®-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

Get tips on using Cellular Senescence Staining Kit to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)