Get tips on using Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using Mid Range DNA Ladder to perform DNA Ladder Medium Range
Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range
Get tips on using Medium Range DNA Ruler to perform DNA Ladder Medium Range
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range
Get tips on using Low Range DNA Ruler Plus to perform DNA Ladder Low Range
Get tips on using Fluorescent Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using Fluorescent Long Range DNA Ladder to perform DNA Ladder Long Range
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment