Protein expression and purification Mammalian cells HEK 293

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Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2

Products Thermo Fisher Scientific ErbB2 (HER-2) Antibody Cocktail

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Products Abcam Anti-Beclin 1 antibody [2A4] (ab114071)

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Products Abcam Anti-Beclin 1 antibody [2A4] (ab114071)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Products Sigma-Aldrich Anti-LC3B antibody produced in rabbit

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Products Sigma-Aldrich Anti-LC3B antibody produced in rabbit

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type HT-29

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Products Sigma-Aldrich Anti-p62/SQSTM1 antibody produced in rabbit

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Autophagy assay cell type - HeLa

Products Abcam Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Autophagy assay cell type - HepG2

Products Abcam Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Get tips on using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] to perform Immunohistochemistry Human - Hes1

Products OriGene HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5]

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