siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using SPLInsert™ Hanging to perform Cell migration / Invasion cell type - HaCat

Get tips on using LC3B antibody to perform Autophagy assay cell type - NRK-52E

Get tips on using TGFβ1 Antibody (TB21): sc-52893 to perform Western blotting TGF-beta1

Get tips on using PR Antibody (AB-52): sc-810 to perform Immunohistochemistry Mouse - PR

Get tips on using Akt1 Antibody (B-1): sc-5298 to perform Western blotting AKT

Get tips on using Oct-3/4 Antibody (C-10): sc-5279 to perform Western blotting Oct4

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - NRK-52E

Get tips on using Anti-Beclin 1 antibody (ab62557) to perform Autophagy assay cell type - NRK-52E

Get tips on using Purified Mouse Anti-IKKγ Clone 54/IKKγ/NEMO (RUO) to perform Western blotting IKKgamma