ChIP H3K27me3 Mouse Sheep

Get tips on using High-Sensitivity ChIP Kit (ab185913) to perform ChIP Mouse - MLL-AF9/NrasG12D AML

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Brain

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - HT22

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - NIH3T3

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Hepa-1

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Ovary

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Podocyte

Get tips on using EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit to perform ChIP Mouse - HSC

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Hepa-1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.