Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - 3T3-L1 cells
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Mouse - 3T3-L1 cells
Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts
Get tips on using High-Sensitivity ChIP Kit (ab185913) to perform ChIP Mouse - MLL-AF9/NrasG12D AML
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Brain
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - HT22
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - NIH3T3
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Hepa-1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment