crispr-human-repression-hpv-16-e7

Get tips on using pLX_311-Cas9 to perform CRISPR Human - Repression AKT2

Get tips on using dCas9 plasmid to perform CRISPR Human - Repression BRCA1

Get tips on using px330-mcherry to perform CRISPR Human - Repression HOTAIR

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT

Get tips on using Maxwell® 16 LEV simplyRNA Purification Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression miR-21

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pCW-Cas9 to perform CRISPR Human - Repression ENII-CP/X

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression HBV

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.