CRISPR Rat Activation

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using MicroRNA isolation kit to perform CRISPR

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Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

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Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α

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Get tips on using hCas9 to perform CRISPR Mouse - Deletion Dck

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Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression

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Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

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Get tips on using lentiCas9-Blast to perform CRISPR Mouse - Deletion RAW 264.7 Casp1

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Get tips on using hCas9 to perform CRISPR Mouse - Deletion C2C12 Dmd

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