Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using PE anti-human CD3 Antibody to perform Flow cytometry Anti-bodies Human - CD3

Get tips on using APC anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69

Get tips on using CD25 Antibody, anti-human, PE to perform Flow cytometry Anti-bodies Human - CD25

Get tips on using APC anti-human CD25 Antibody to perform Flow cytometry Anti-bodies Human - CD25

Get tips on using CD49d Antibody, anti-human, Biotin to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using PE anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using CD127 Antibody, anti-human, Biotin to perform Flow cytometry Anti-bodies Human - CD127

Get tips on using FITC anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Get tips on using APC anti-human CD24 Antibody to perform Flow cytometry Anti-bodies Human - CD24

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.