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Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brain

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Whole Mouse Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using RNeasy DSP FFPE Kit to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - human placental tissue

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - human liver tissue