DNA isolation / purification Cells Immortalized cell lines

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using LC3B Antibody to perform Autophagy assay cell type - Rat cerebral coritcal tissue

Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using Fluoro hROS to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human gastric organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse skin organoids

Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse prostate organoid