RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Vibrio cholerae

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Pseudomonas aeruginosa

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Shigella flexneri

Get tips on using HiSpeed Plasmid Kits to perform Plasmid Isolation Bacillus group

Get tips on using QuickLyse Miniprep Kit (250) to perform Plasmid Isolation Enterobacteriaceae

Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4