Protein Expression Prokaryotic cells E. coli

Get tips on using MboI (10 U/µL) to perform Restriction Enzymes MboI

Get tips on using MboII (5 U/µL) to perform Restriction Enzymes MboII

Get tips on using AvrII NEB#R0174 to perform Restriction Enzymes BlnI / AvrII / XmaJI

Get tips on using NcoI (10 U/µL) to perform Restriction Enzymes NcoI

Get tips on using PvuII (10 U/µL) to perform Restriction Enzymes PvuII

Get tips on using PvuI (10 U/µL) to perform Restriction Enzymes PvuI

Get tips on using BglI (10 U/µL) to perform Restriction Enzymes BglI

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse ear tissue

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.