siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Get tips on using Tryptic Soy Broth for microbiology to perform Bacterial cell culture media Salmonella Typhi

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform cDNA synthesis Cell lines

Get tips on using MicroSEQ™ D2 rDNA Fungal PCR Kit to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using Fast MicroSEQ™ D2 Fungal rDNA PCR Kit to perform Cell Culture Contamination Detection Kit Fungi

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using miRCURY Exosome Serum/Plasma Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - MCF-7