Immunohistochemistry Anti-mouse IgG

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Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Products MBL international corporation Anti-LC3 (Rat) pAb

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Products MBL international corporation Anti-LC3 (Rat) pAb

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K4me2

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K9me3

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K36me1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K36me3

Get tips on using Recombinant Anti-PCNA antibody [EPR3821] (ab92552) to perform Western blotting PCNA

Products Abcam Recombinant Anti-PCNA antibody [EPR3821] (ab92552)

Get tips on using Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) to perform Western blotting PARP

Products Abcam Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315)

Get tips on using Recombinant Anti-Bax antibody [E63] (ab32503) to perform Western blotting Bax

Products Abcam Recombinant Anti-Bax antibody [E63] (ab32503)

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Western blotting SOX9

Products Abcam Recombinant Anti-SOX9 antibody [EPR14335] (ab185230)

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