When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using StemSep™ Human CD34 Positive Selection Cocktail to perform Cell Isolation CD34+ cells
Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte
Get tips on using EasySep™ Release Human CD19 Positive Selection Kit to perform Cell Isolation B cell
Get tips on using EasySep™ Human CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells
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