Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using siRNA MAPKAPK-2 to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Cox-2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 COX-2

Get tips on using pPIC9K-duIL-2 to perform Protein Expression Eukaryotic cells - P. pastoris rduIL-2

Get tips on using Dlx-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 DLX-2

Get tips on using Bcl-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 bcl-2

Get tips on using EMBacY#2 to perform Protein Expression Eukaryotic cells - Hi5 hcAbl

Get tips on using pDDGFP-2 to perform Protein Expression Eukaryotic cells - S. cerevisiae GPCRs

Get tips on using SOX-2 (SP76) Rabbit Monoclonal Antibody to perform Immunohistochemistry Human - SOX2