siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using pET3a-hsEH to perform Protein Expression Prokaryotic cells - E. coli hsEH

Get tips on using Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix to perform 3D Cell Culture Media Mouse fallopian organoids

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - BxPC-3

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecium

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #13082 to perform Autophagy assay cell type - RAW 264.7

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - PANC-1

Get tips on using HeV NCORE pET43.1a to perform Protein Expression Prokaryotic cells - E. coli HeV N

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?