siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Get tips on using HSP60 monoclonal antibody (LK-1) to perform Western blotting Hsp60

Get tips on using HSP70 ELISA Kit (High-Sensitivity) to perform ELISA Mouse - HSP70

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Rat - HSP70

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hSSCs

Get tips on using HSP 60 Antibody (C-10): sc-376240 to perform Western blotting Hsp60

Get tips on using REPLI-g Single Cell Kit (96) to perform Whole Genome Amplification Cell lines

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12