RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using beta Catenin Monoclonal Antibody (CAT-5H10) to perform Western blotting eta-catenin
Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Salmonella enterica serovar Indiana (S. Indiana)
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Eye
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Esophagus
Get tips on using PowerSoil® DNA isolation to perform DNA isolation / purification Bacteria - Gram positive Enterococcus faecium
Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Activation 3T3-L1 eIF5A1
Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Human - Activation ERαY537S
Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression HPV-18 E6
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment