Protein Expression Eukaryotic cells P. pastoris

Get tips on using Promega PCR Master Mix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using PureYield™ Plasmid Midiprep System to perform Plasmid Isolation Streptococcus pneumoniae

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Repression GLT25D1

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Promega PCR Master Mix to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Deletion NOX4

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Mouse - Deletion L929 SIRT2