Flowcytometry CD3 Mouse / IgG1, kappa

Get tips on using Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO) to perform Immunohistochemistry Human - MLH1

Get tips on using Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441 to perform Immunohistochemistry Human - AR

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8 to perform Immunohistochemistry Human - CK20

Get tips on using Purified Mouse Anti-IKKγ Clone 54/IKKγ/NEMO (RUO) to perform Western blotting IKKgamma

Get tips on using Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody to perform Western blotting AKT

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Rat - HSP70

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using Monoclonal ANTI-FLAG® M2 antibody produced in mouse to perform ChIP Anti-bodies FLAG