siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform Stem cell Differentiation media hDPSCs differentiation into adipogenic cells

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform Stem cell Differentiation media hDPSCs differentiation into osteogenic cells

Get tips on using MEMα with L-Glutamine, Phenol Red, Sodium Pyruvate and Nucleosides to perform Stem cell Differentiation media mPericytes differentiation into Osteogenic cells

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using Gibco™KnockOut™ DMEM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using Gibco™KnockOut™ DMEM/F-12 to perform Stem cell Differentiation media hiPSCs or hPSCs differentiation into trophoblasts

Get tips on using STEMdiff™ APEL™ 2-LI Medium to perform Stem cell Differentiation media hESCs or hiPSCs differentiation into Cardiomyocytes

Get tips on using Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides to perform 3D Cell Culture Media hiPSC-derived cardiac organoids

Get tips on using anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using IMAGEN™ Herpes Simplex Virus (HSV) Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus