siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

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A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Cellular assays Necrosis SK-BR-3

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Products Sigma-Aldrich Imprint® Methylated DNA Quantification Kit

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - BHK-21

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - mouse iPSC

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - mouse splenocytes

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

Get tips on using FitAmp Blood and Cultured Cell DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Products Epigentek FitAmp Blood and Cultured Cell DNA Extraction Kit

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

DNA Whole Genome Amplification Cell lines

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages

Products Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Mice spermatogonial stem cells (mSSCs)(C18-4)

Products Thermo Fisher Scientific Gibco™DMEM/F-12

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