siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells

Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - K562 cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized Caov-3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SHG-44

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDAH 2774