RNA isolation / purification Tissue Human

Get tips on using Rabbit anti-calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34

Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

Get tips on using FOXP3 Rabbit Polyclonal Antibody to perform Western blotting FOXP3

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.