siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Hepa-1c1c7

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Mammalian cells - HeLa

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - mouse bone marrow-derived macrophages

Get tips on using GeneRead DNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using GenElute™ Plasmid Miniprep Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform