DNA methylation profiling Gene specific profiling Caco-1

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Rat - Endothelin 1

Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - NRK-49F

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - BRL-3A

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.