siRNA / miRNA gene silencing Human BCP-1

Get tips on using Silencer® FANCD2 siRNA (human) to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2

Get tips on using ON-TARGETplus Human PPRC1 siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PRC (PGC-1α–related coactivator)/PPRC1

Get tips on using Hs_BAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H BAP1_5

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - MSTO-211H BAP1_3

Get tips on using Hs_BAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - NCI-H460 BAP1_5

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - NCI-H460 BAP1_3

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - NCL-H40 BAP1_3

Get tips on using Hs_BAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 BAP1_5

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 BAP1_3

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.