Protein Expression Prokaryotic cells A. cellulolyticus

Get tips on using ApaLI restriction enzyme to perform Restriction Enzymes ApaLI / Alw44I

Get tips on using ApaLI NEB#R0507 to perform Restriction Enzymes ApaLI / Alw44I

Get tips on using Anti-GATA4 Antibody to perform Western blotting GATA-4

Get tips on using Agencourt AMPure XP - PCR Purification to perform

Get tips on using Ki67/MKI67 Antibody [Allophycocyanin] to perform Immunohistochemistry Mouse - Ki67

Get tips on using NOTCH1/activated Notch1 Antibody to perform Immunohistochemistry Mouse - Notch1

Get tips on using AfeI NEB#R0652 to perform Restriction Enzymes AfeI / Eco47III / Aor51HI

Get tips on using Anti-Smad4/DPC4 Antibody to perform Western blotting Smad4

Get tips on using siRNA AURKA to perform siRNA / miRNA gene silencing Human - A549 AURKA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.