Immunohistochemistry 53BP2 phospho (ser-25) Rabbit IgG Human

Get tips on using Anti-LC3B antibody (ab51520) to perform Immunohistochemistry Mouse - LC3

Get tips on using VEGF A - AB-90040 to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Insulin (Autostainer Link 48) to perform Immunohistochemistry Mouse - Insulin

Get tips on using NOTCH1/activated Notch1 Antibody to perform Immunohistochemistry Mouse - Notch1

Get tips on using Lysozyme EC 3.2.1.17 (Concentrate) to perform Immunohistochemistry Mouse - Lysozyme

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Anti-MUC2 antibody (ab76774) to perform Immunohistochemistry Mouse - Muc-2