Protein Expression Eukaryotic cells Iranian lizard Leishmania cells

Get tips on using pBESTBL1181:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pBIN61:α-AIC3 to perform Protein Expression Prokaryotic cells - A. tumefaciens α-amylase inhibitor

Get tips on using MrNV-pGEX-6P-1 to perform Protein Expression Prokaryotic cells - E. coli MrNV capsid

Get tips on using pIRES2-EGFP-PBD-1 to perform Protein Expression Prokaryotic cells - E. coli PBD1-EGFP

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1

Get tips on using pTRAkc-AH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Maj-NPLP to perform Protein Expression Prokaryotic cells - E. coli M. japonicus neuroparsin-like peptide