Protein Expression Prokaryotic cells C. crescentus JS1014

Get tips on using Quick Amp Labeling Kit-one color to perform RNA amplification & labeling Mammalian - RNA, Mice Cochlaea Cyanine CTP

Get tips on using Anti-Beta Catenin CTNNB1 Antibody Picoband™ (Monoclonal, 1F6) to perform Immunohistochemistry Human - β-catenin

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Anti-LGR5 mouse mAb, clone OTI2A2, PE conjugated to perform Flow cytometry Anti-bodies Human - LGR5

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - THP-1

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Get tips on using Monoclonal Mouse Anti-Human CA 125 (Dako Omnis) Clone M11 to perform Immunohistochemistry Human - CA125

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 G3BP