RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using PL_MmHsp90b1 to perform Protein Expression Eukaryotic cells - CHO Hsp90b1

Get tips on using PL_MmEro1l to perform Protein Expression Eukaryotic cells - CHO Ero1l

Get tips on using PL_MmBax to perform Protein Expression Eukaryotic cells - CHO Bax

Get tips on using PL_MmBak1 to perform Protein Expression Eukaryotic cells - CHO Bak1

Get tips on using PL_MmAtf4 to perform Protein Expression Eukaryotic cells - CHO Atf4

Get tips on using CpGfree to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using CpGrich to perform Protein Expression Eukaryotic cells - CHO EGFP

Get tips on using OneDay ChIP kit to perform ChIP Human - Kupffer Cells

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using QuantiTect Virus Kit to perform RNA quantification qPCR