siRNA / RNAi /miRNA transfection Human Cells KG-1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - MEL-1220

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - PC-12

Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - INS-1E

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - PC-12

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using 100 bp DNA ladder-TLAD-100 to perform DNA Ladder 100 bp

Get tips on using GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker to perform DNA Ladder 100 bp

Get tips on using paxillin Antibody (177): sc-136297 to perform Western blotting Paxillin

Get tips on using RhoA Monoclonal Antibody (1B8-1C7) to perform Western blotting RhoA