ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using AMPIPROBE® Candida 1 Assay Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - MCF 10A

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using Anti-Collagen VII antibody [LH7.2] to perform Immunohistochemistry Collagen VII antibody [LH7.2] - Mouse Human -NA-

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse kidney

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.