Protein Expression Eukaryotic cells CHO

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using rMaj-pCHH-B to perform Protein Expression Prokaryotic cells - E. coli M. japonicus CHH-like peptide

Get tips on using Maj-NPLP to perform Protein Expression Prokaryotic cells - E. coli M. japonicus neuroparsin-like peptide

Get tips on using pET-PvD1 to perform Protein Expression Prokaryotic cells - E. coli PvD1, from Phaseolus vulgaris L.

Get tips on using pET-28a(+)-g-α13 to perform Protein Expression Prokaryotic cells - E. coli α-13 giardin

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized CHO

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pTip-QC2-gi_21221697 to perform Protein Expression Prokaryotic cells - R. erythropolis putative gntR-family regulator

Get tips on using pSS1-EBA140RIII–V to perform Protein Expression Prokaryotic cells - L. lactis EBA140RIII–V P. falciparum

Get tips on using pTRAkc-rbcs1-cTP/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp