RNA isolation / purification Tissue Human

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Brainstem

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Human Bone

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Bladder

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Brain

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Adipose

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using RNAprep pure Kit to perform RNA isolation / purification Tissue - Human Placenta

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Thyroid gland