siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using PCNA Antibody to perform Western blotting PCNA

Get tips on using GenCatch™ Total RNA Extraction Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using GenCatch™ Total RNA Extraction Kit to perform RNA isolation / purification Tissue - rat liver tissue

Get tips on using Recombinant Anti-PCNA antibody [EPR3821] (ab92552) to perform Western blotting PCNA

Get tips on using miScript PreAMP PCR Kit (60) to perform PCR Preamplification of cDNA - FFPE samples

Get tips on using REDExtract-N-Amp™ PCR ReadyMix™ to perform PCR Mouse

Get tips on using PCNA (D3H8P) XP® Rabbit mAb #13110 to perform Immunohistochemistry Mouse - PCNA

Get tips on using MinElute PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb