siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using pGS-21a Vector to perform Protein expression and purification Bacteria - Escherichia coli p19

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ

Get tips on using ERα Antibody (F-10): sc-8002 to perform Western blotting Estrogen Receptor

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Esophagus

Get tips on using pGEX-6P-1 Vector to perform Protein expression and purification Bacteria - Escherichia coli FleN

Get tips on using pQE-30 Xa Vector to perform Protein expression and purification Bacteria - Escherichia coli Siva1

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.