siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using Blu11 (BlueAQUA) Prestained Protein Ladder（10 to 180 kDa） to perform Protein Ladder Prestained

Get tips on using Blu10 (BlueRAY) Prestained Protein Ladder（10 to 180 kDa） to perform Protein Ladder Prestained

Get tips on using PageRuler™ Prestained Protein Ladder, 10 to 180 kDa to perform Protein Ladder Prestained

Get tips on using Bcl-11B (D6F1) XP® Rabbit mAb #12120 to perform ChIP Anti-bodies CtIP/BCL11A

Get tips on using CD137 (4-1BB) Monoclonal Antibody (17B5), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD137

Get tips on using CD137 (4-1BB) Monoclonal Antibody (17B5), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD137

Get tips on using Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070 to perform Protein Ladder Prestained

Get tips on using Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027) to perform Protein Ladder Prestained

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.