siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Get tips on using BRAIN HEART INFUSION AGAR to perform Bacterial cell culture media Streptococcus pneumoniae

Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Streptococcus pyogenes

Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Listeria monocytogens

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In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Neogen-Brain-Heart Infusion Broth to perform Bacterial cell culture media Salmonella enterica

Get tips on using BHI-BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Listeria monocytogens

Get tips on using Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix to perform 3D Cell Culture Media Mouse fallopian organoids

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