siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

RNA quantification for appropriate concentration and quality (260/280 ratio) is an important step before downstream analysis (including sequencing, RT-qPCR, etc.). Having insufficient RNA quantities or a high salt or phenol in the RNA product can lead to variable or irreproducible downstream results. The various methods used for RNA quantification include: 1. UV spectrophotometric (challenges include: low sensitivity, cannot distinguish between nucleic acid species), 2. Fluorescence-based (challenges include: requires standards, cannot measure amplifiability, not sequence-specific), and 3. RT-PCR (challenges include: requires standards, time-intensive, costly). In order to overcome these challenges, and also to ensure the proper quantification and quality control for RNA product, it is important to use at least two or more methods in order to discard any inconsistencies. Using standards for calibrations increases the sensitivity range for RNA detention (fluorescence- and RT-PCR-based methods). When using RT- PCR, it is important to choose correct primers, aligning to the desired site on the template and of appropriate product length, along with positive, negative and loading controls. It is also important to have at least two primer pairs in order to confirm results.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized U-2 OS

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-468

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion ES (embryonic stem) cells Slx2

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Deletion ES (embryonic stem) cells MIR